Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?

被引:4
|
作者
Mardani-Kataki, Masoumeh [1 ,2 ]
Beiromvand, Molouk [1 ,2 ]
Teimoori, Ali [3 ,4 ,5 ]
Amari, Afshin [6 ,7 ]
Tavalla, Mehdi [1 ,2 ]
机构
[1] Ahvaz Jundishapur Univ Med Sci, Infect & Trop Dis Res Ctr, Hlth Res Inst, Ahvaz, Iran
[2] Ahvaz Jundishapur Univ Med Sci, Fac Med, Sch Med, Dept Parasitol, Ahvaz, Iran
[3] Ahvaz Jundishapur Univ Med Sci, Canc Res Ctr, Ahvaz, Iran
[4] Ahvaz Jundishapur Univ Med Sci, Dept Virol, Ahvaz, Iran
[5] Hamadan Univ Med Sci, Fac Med, Dept Virol, Hamadan, Iran
[6] Ahvaz Jundishapur Univ Med Sci, Cellular & Mol Res Ctr, Med Basic Sci Res Inst, Ahvaz, Iran
[7] Ahvaz Jundishapur Univ Med Sci, Sch Med, Dept Immunol, Ahvaz, Iran
关键词
Immuno-PCR; ELISA; Toxoplasma gondii; Sensitivity; Specificity; WESTERN-BLOT; INFECTION; SEROPREVALENCE; OOCYSTS; ASSAY; PROTEINS; ANTIGENS; CATS; GRA7;
D O I
10.1007/s11686-022-00537-1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Introduction IgG antibodies against T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-linked immunosorbent assay (ELISA) methods for detection of T. gondii IgG antibody. Methods Immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against T. gondii were measured by the ELISA method in 81 participants. In addition, detection of acute and chronic toxoplasmosis was performed via the ELISA IgG avidity. The set-up of iPCR was carried out and then, serum IgG of subjects were detected using the iPCR method. Results Of 81 samples, 4 (4.9%) and 30 (37%) cases were be found positive for IgM and IgG against T. gondii in the ELISA method, respectively. Moreover, of 81 specimens, 42 (51.9%) and 39 (48.1%) samples had low-avidity IgG and high-avidity IgG by the IgG avidity kit, respectively. While, 59 (72.8%) of 81 samples were detected positive using the iPCR technique. Kappa (kappa) value coefficient, between the iPCR and ELISA (for IgG) showed a strong agreement (0.360, p value < 0.001). A value of 0.25 I.U./ml for serum IgG [area under curve (AUC) = 0.720 (CI = 0.613-0.827); p = 0.002] was the cut-off value to differentiating between positive and negative toxoplasmosis (with sensitivity 66.0% and specificity 60.0%). Conclusion Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. gondii IgG antibody. However, more complementary investigations are widely needed in this regard.
引用
收藏
页码:904 / 911
页数:8
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