HpRNAs Derived from Different Regions of the NIb Gene Have Different Abilities to Protect Tobacco from Infection with Potato Virus Y

被引：16
|
作者：
Sun, Zhao-Nan [1 ]
Song, Yun-Zhi [1 ]
Yin, Guo-Hua [1 ]
Zhu, Chang-Xiang [1 ]
Wen, Fu-Jiang [1 ]
机构：
[1] Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
来源：
JOURNAL OF PHYTOPATHOLOGY | 2010年 / 158卷 / 7-8期
基金：
中国国家自然科学基金;
关键词：
prokaryotic expression; hpRNA; RNA-mediated virus resistance; Potato virus Y; RNA; INTERFERENCE; RESISTANCE;
D O I：
10.1111/j.1439-0434.2009.01650.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
RNA interference (RNAi) involves the degradation of homologous mRNA sequences in organisms and is induced by double-stranded RNA (dsRNA). We have constructed three hairpin RNA (hpRNA) prokaryotic expression vectors derived from the 5' region, the middle region and the 3' region of the Nuclear inclusion protein b (NIb) gene, and then expressed these in M-JM109lacY. Resistance analyses and Northern blotting showed that different hpRNAs had different abilities to protect tobacco plants from infection with Potato virus Y (PVY) and that this resistance was RNA-mediated.
引用
收藏
页码：566 / 568
页数：3
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