A genetic screen for mutations affecting embryonic development in medaka fish (Oryzias latipes)

被引:97
|
作者
Loosli, F [1 ]
Köster, RW [1 ]
Carl, M [1 ]
Kühnlein, R [1 ]
Henrich, T [1 ]
Mücke, M [1 ]
Krone, A [1 ]
Wittbrodt, J [1 ]
机构
[1] Max Planck Inst Biophys Chem, SFB 271 Junior Grp, D-37077 Gottingen, Germany
关键词
medaka fish; Oryzias latipes; genetic screen; embryonic development; eye development;
D O I
10.1016/S0925-4773(00)00406-8
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In a pilot screen, we assayed the efficiency of ethyinitrosourea (ENU) as a chemical mutagen to induce mutations that lead to early embryonic and larval lethal phenotypes in the Japanese medaka fish, Oryzias latipes. ENU acts as a very efficient mutagen inducing mutations at high rates in germ cells. Three repeated treatments of male fish in 3 mM ENU for 1 h results in locus specific mutation rates of 1.1-1.95 X 10(-3). Mutagenized males were outcrossed to wild type females and the Fl offspring was used to establish F2 families. F2 siblings were intercrossed and the F3 progeny was scored 24, 48 and 72 h after fertilization for morphological alterations affecting eye development. The presented mutant phenotypes were identified using morphological criteria and occur during early developmental stages of medaka. They are stably inherited in a Mendelian fashion. The high efficiency of ENU to induce mutations in this pilot screen indicates that chemical mutagenesis and screening for morphologically visible phenotypes in medaka fish allows the genetic analysis of specific aspects of vertebrate development complementing the screens performed in other vertebrate model systems. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:133 / 139
页数:7
相关论文
共 50 条
  • [41] Effects of copper and cadmium spiked-sediments on embryonic development of Japanese medaka (Oryzias latipes)
    Barjhoux, Iris
    Baudrimont, Magalie
    Morin, Benedicte
    Landi, Laure
    Gonzalez, Patrice
    Cachot, Jerome
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2012, 79 : 272 - 282
  • [42] Factors affecting the uptake of DMSO by the eggs and embryos of medaka, Oryzias latipes
    Routray, P
    Suzuki, T
    Strüssmann, CA
    Takai, R
    THERIOGENOLOGY, 2002, 58 (08) : 1483 - 1496
  • [43] Characterization and expression of embryonic and adult globins of the teleost Oryzias latipes (Medaka)
    Maruyama, K
    Yasumasu, S
    Iuchi, I
    JOURNAL OF BIOCHEMISTRY, 2002, 132 (04): : 581 - 589
  • [44] High incidence of mosaic mutations induced by irradiating paternal germ cells of the medaka fish, Oryzias latipes
    Shimada, A
    Shima, A
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2001, 495 (1-2) : 33 - 42
  • [45] ras oncogene mutations in diethylnitrosamine-induced hepatic tumors in medaka (Oryzias latipes), a teleost fish
    Liu, Z
    Kullman, SW
    Bencic, DC
    Torten, M
    Hinton, DE
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2003, 539 (1-2) : 43 - 53
  • [46] Ovarian Germline Stem Cells in the Teleost Fish, Medaka (Oryzias latipes)
    Nakamura, Shuhei
    Kobayashi, Kayo
    Nishimura, Toshiya
    Tanaka, Minoru
    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2011, 7 (04): : 403 - 409
  • [47] GROWTH AND SOCIAL-BEHAVIOR IN THE FISH ORYZIAS-LATIPES (MEDAKA)
    RUZZANTE, DE
    DOYLE, RW
    AMERICAN ZOOLOGIST, 1989, 29 (04): : A86 - A86
  • [48] Developmental biology of medaka fish (Oryzias latipes) exposed to alkalinity stress
    Yao, Z. L.
    Lai, Q. F.
    Zhou, K.
    Rizalita, R. -E.
    Wang, H.
    JOURNAL OF APPLIED ICHTHYOLOGY, 2010, 26 (03) : 397 - 402
  • [49] Diversification of olfactory receptor genes in the Japanese medaka fish, Oryzias latipes
    Kondo, R
    Kaneko, S
    Sun, H
    Sakaizumi, M
    Chigusa, SI
    GENE, 2002, 282 (1-2) : 113 - 120
  • [50] AVOIDANCE BY OLFACTION IN A FISH, MEDAKA (ORYZIAS-LATIPES), TO AQUATIC CONTAMINANTS
    HIDAKA, H
    TATSUKAWA, R
    ENVIRONMENTAL POLLUTION, 1989, 56 (04) : 299 - 309