Clinicopathological parameters and prognostic relevance of miR-21 and PTEN expression in Wilms' tumor

Background: MiR-21 is one of the most often found miRNAs overexpressed in solid tumors, while PTEN is the most highly mutated tumor suppressor gene. Our purpose was to examine the expression levels of miR-21 and PTEN protein in Wilms' tumor (WT) and in para-tumoral tissues and to investigate the relationships among miR-21, PTEN expression, clinicopathological parameters and the prognosis of patients withWT. Methods: The expression levels of miR-21 and PTEN protein inWTand corresponding para-tumoral tissueswere investigated by qRT-PCR and Western blot, respectively. Differences in patient survival were determined using the Kaplan-Meier method and the log-rank test. A Cox proportional hazards regression analysis was used for univariate and multivariate analyses of prognostic values. Results: Comparedwith para-tumoral renal tissues, the expression levels of miR-21were significantly upregulated in WT tissues, while the PTEN protein were significantly downregulated (P < 0.05). Analyses of the clinicopathological parameters showed that themiR-21 expression level was significantly associated with age, late clinical stage, histopathological tumor type and lymphatic metastasis (P < 0.05). PTEN protein expression was significantly associated with age, late clinical stage and histopathological tumor type (P < 0.05). The univariate linear regression analysis illustrated a significant negative correlation between miR-21 and PTEN expression (r = -0.687, P < 0.05). The Kaplan-Meier curve showed that patients with highmiR-21 and lowPTEN protein expression survived significantly longer (P < 0.05). However, amultivariate analysis suggested that neither the expression level ofmiR-21 nor that of PTEN is an independent prognostic factor for overall survival. Conclusions: Both upregulated miR-21 and downregulated PTEN expression have a possible correlation with the aggressive progression and poor prognosis of WT, which suggests that upregulated miR-21 and downregulated PTEN expression may be valuable markers of tumor progression and indicators of the prognosis ofWT. (C) 2017 Elsevier Inc. All rights reserved.