Enantiomer separation of 1-O-alkylglycerols by HPLC on a chiral slurry-packed capillary column

Almost complete enantiomer separations of racemic 1-O-alkylglycerols as their bis(3,5-dinitrophenylurethane) derivatives were achieved by HPLC using a. slurry-packed fused-silica capillary (40 cm x 0.32 mm i.d.) with a chiral phase, N-(S)-2-(4-chlorophenyl) isovaleroyl-D-phenylglycine, ionically bonded to 5 mum silica gel. The analysis was optimized by using a ternary mobile phase, n-hexane-1,2-dichloroethane-ethanol, and UV detection at 254 nm.