A new method of determination of diffusion coefficients using capillary zone electrophoresis (peak-height method)

A new method of the determination of the diffusion coefficients, D, called "the peak-height method" has been developed. When the zone broadening of a species is solely caused by axial molecular diffusion, its diffusion coefficient can be calculated. The diffusion coefficients of L-cysteine and glutathione in a neutral phosphate buffer were measured by this method. Comparison between the experimental diffusion coefficient and the one calculated by the Wilke-Chang equation shows that the method gives good results.