Stimulation of Bacillus sp. by lipopeptide biosurfactant for the degradation of aromatic amine 4-Chloroaniline

This study aims to reveal that the biosurfactant act as a stimulant in aromatic amine 4-Chloroaniline (4-CA) degradation. Isolated degrading strain Bacillus sp. was used for the production of biosurfactant with help of substrate such as engine oil. The surfactant production by the strain was studied by using various screening methods and the results showed best emulsification activity (75%), surface tension reduction activity (28.6 mNm(-1)) and oil spreading activity (5.9 cm). The obtained surfactant was characterized using Fourier transform infrared spectroscopy (FT-IR), Gas chromatography-Mass Spectrometry (GC-MS), Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) which confirmed that the nature of surfactant is lipopeptide. The maximum removal of 4-CA was achieved in different environmental conditions at concentration 100 mg L-1, neutral pH and temperature 30 degrees C. In the degradation studies, the 4-CA was removed upto 76% by Bacillus sp but in the presence of lipopeptide surfactant, the Bacillus sp removed 4-CA upto 100%. The degraded metabolites were further characterized using High-Pressure Liquid Chromatography (HPLC) and GC-MS. This research indicated that strain Bacillus sp along with the lipopeptide biosurfactant possesses higher potential in the bioremediation of 4-CA compound from the environment.