Isolation and characterization of Indian isolates of camel pox virus

被引：20

作者：

Bhanuprakash, V. ^{[1
]}

Balamurugan, V. ^{[1
,2
]}

Hosamani, M. ^{[1
,3
]}

Venkatesan, G. ^{[1
]}

Chauhan, Bina ^{[1
,4
]}

Srinivasan, V. A. ^{[1
,5
]}

Chauhan, R. S. ^{[1
,6
]}

Pathak, K. M. L. ^{[1
,7
]}

Singh, Raj Kumar ^{[1
,8
]}

机构：

[1] Indian Vet Res Inst, Pox Virus Dis Lab, Div Virol, Naini Tal 263138, Uttarakhand, India

[2] Project Directorate Anim Dis Monitoring & Surveil, Bangalore 560024, Karnataka, India

[3] Indian Vet Res Inst, Bangalore 560024, Karnataka, India

[4] Indian Vet Res Inst, Div Biol Prod, Izatnagar 243122, Uttar Pradesh, India

[5] Indian Immunol Ltd, Hyderabad 500032, Andhra Pradesh, India

[6] Deemed Univ, Indian Vet Res Inst, Izatnagar 243122, Uttar Pradesh, India

[7] Natl Res Ctr Camel, Bikaner, Rajasthan, India

[8] Natl Res Ctr Equines, Hisar 125001, Haryana, India

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2010年 / 42卷 / 06期

关键词：

Camel pox; Virus isolation; Identification; Characterization; PCR; Sequence analysis;

D O I：

10.1007/s11250-010-9560-z

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains.

引用

页码：1271 / 1275

页数：5

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