Genetic structure of an introduced pest, grape phylloxera (Daktulosphaira vitifoliae Fitch), in Europe

被引:47
|
作者
Forneck, A [1 ]
Walker, MA
Blaich, R
机构
[1] Univ Hohenheim, Inst Obst Gemuse & Weinbau, Fachgebiet Weinbau, D-70593 Stuttgart, Germany
[2] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA
关键词
Daktulosphaira vitifoliae; aphid; genetic structure; AFLP-PCR;
D O I
10.1139/gen-43-4-669
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A model for the genetic structure of grape phylloxera populations in Europe was developed using hierarchical sampling techniques and AFLP-PCR (amplified fragment length polymorphism - polymerase chain reaction) methodology. One-hundred three European and 6 North American phylloxera populations were studied. An additional European sampling set comprising 60 samples was analyzed to study regional subdivision. The populations grouped into two clusters loosely correlated with collection site location. Phylloxera populations collected from northern (above lat 43 degrees) geographic regions were significantly different from southern (below 43 degrees) populations. The northern cluster was more heterogeneous than the southern cluster, possibly reflecting holocyclic versus anholocyclic reproduction. Microgeographic scales of phylloxera genetic structure displayed as much variation within as among host plants. The host plant did not affect the genetic structure of European phylloxera as revealed in two independent experiments.
引用
收藏
页码:669 / 678
页数:10
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