Polyamine depletion protects HL-60 cells from 2-deoxy-D-ribose-induced apoptosis

被引：23
|
作者：
Monti, MG
Ghiaroni, S
Pernecco, L
Barbieri, D
Marverti, G
Franceschi, C
机构：
[1] Dipartimento Sci Biomed, Sez Chim Biol, I-41100 Modena, Italy
[2] Dipartimento Sci Biomed, Sez Patol Gen, I-41100 Modena, Italy
来源：
LIFE SCIENCES | 1998年 / 62卷 / 09期
关键词：
polyamines; apoptosis; 2-deoxy-D-ribose; HL-60; cells;
D O I：
10.1016/S0024-3205(97)01181-8
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We investigated the involvement of natural polyamines in HL-60 cell death triggered by exposure to 2-deoxy-D-ribose (dRib). In contrast to previous studies, exogenous polyamines failed to protect HL-60 cells against apoptosis caused by dRib. Moreover, in our experimental conditions, depletion of intracellular levels of putrescine and spermidine by alpha-difluoromethylornithine (DFMO) delayed the onset of apoptosis by at least a day or so. Exogenous polyamines reversed the beneficial effect of DFMO and restored the apoptotic levels observed in dRib-treated cells. We suggested that polyamines, especially putrescine and spermidine, act as facilitating factors in the induction of apoptosis triggered by dRib in HL-60 cells.
引用
收藏
页码：799 / 806
页数：8
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