Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid

被引:2
|
作者
Truong, Jia Q. [1 ]
Nguyen, Stephanie [2 ]
Bruning, John B. [2 ]
Shearwin, Keith E. [1 ]
机构
[1] Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia
[2] Univ Adelaide, Sch Biol Sci, Inst Photon & Adv Sensing IPAS, Adelaide, SA 5005, Australia
基金
澳大利亚研究理事会;
关键词
crystallography; experimental phasing; B4C; tetrabromoterephthalic acid; lysozyme; co-crystallization; CRYSTAL-STRUCTURE; MAGIC TRIANGLE; MACROMOLECULAR CRYSTALLOGRAPHY;
D O I
10.1107/S2053230X21004052
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The phase problem is a persistent bottleneck that impedes the structure-determination pipeline and must be solved to obtain atomic resolution crystal structures of macromolecules. Although molecular replacement has become the predominant method of solving the phase problem, many scenarios still exist in which experimental phasing is needed. Here, a proof-of-concept study is presented that shows the efficacy of using tetrabromoterephthalic acid (B4C) as an experimental phasing compound. Incorporating B4C into the crystal lattice using co-crystallization, the crystal structure of hen egg-white lysozyme was solved using MAD phasing. The strong anomalous signal generated by its four Br atoms coupled with its compatibility with commonly used crystallization reagents render B4C an effective experimental phasing compound that can be used to overcome the phase problem.
引用
收藏
页码:156 / 162
页数:7
相关论文
共 4 条
  • [1] Crystals of the ribonucleotide reductase R2 protein from Chlamydia trachomatis obtained by heavy-atom co-crystallization
    Stenmark, P
    Högbom, M
    Roshick, C
    McClarty, G
    Nordlund, P
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 376 - 378
  • [2] Heavy-atom Database System:: a tool for the preparation of heavy-atom derivatives of protein crystals based on amino-acid sequence and crystallization conditions
    Sugahara, M
    Asada, Y
    Ayama, H
    Ukawa, H
    Taka, H
    Kunishima, N
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2005, 61 : 1302 - 1305
  • [3] Crystallization and heavy-atom derivatization of StHsp14.0, a small heat-shock protein from Sulfolobus tokodaii
    Hayashi, Takuro
    Abe, Tetsuya
    Takeda, Kazuki
    Akiyama, Nobuhiko
    Yohda, Masafumi
    Miki, Kunio
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2009, 65 : 1007 - 1010
  • [4] Equilibrating parent aminomercaptocarbene and CO2 with 2-amino-2-thioxoacetic acid via heavy-atom quantum tunneling
    Bernhardt, Bastian
    Schauermann, Markus
    Solel, Ephrath
    Eckhardt, Andre K.
    Schreiner, Peter R.
    CHEMICAL SCIENCE, 2022, 14 (01) : 130 - 135