Fetal gene transfer with lentiviral vectors: long-term in vivo follow-up evaluation in a rat model

被引：13

作者：

Toelen, Jaan ^{[1
]}

Deroose, Christophe M. ^{[1
]}

Gijsbers, Rik ^{[1
]}

Reumers, Veerle ^{[1
]}

Sbragia, Lourenco N. ^{[1
]}

Vets, Sofie ^{[1
]}

Chitneni, Satish K. ^{[1
]}

Bormans, Guy ^{[1
]}

Mortelmans, Luc ^{[1
]}

Deprest, Jan A. ^{[1
]}

Debyser, Zeger ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Med, Ctr Surg Technol, B-3000 Louvain, Belgium

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2007年 / 196卷 / 04期

关键词：

fetal intervention; gene therapy; lentiviral vector; noninvasive imaging;

D O I：

10.1016/j.ajog.2007.01.038

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: The purpose of this study was to evaluate the long-term expression of a transgene and subsequent immune response after the injection of lentiviral vectors in a fetal rats. STUDY DESIGN: Fetal rats were injected in the liver, peritoneal cavity, or lung at E19 ( term, E21) with a lentiviral vector expressing enhanced green fluorescent protein and luciferase. Controls received saline solution. After birth, full body bioluminescence was done at weeks 1, 4, 10, and 30 of life; seroconversion for the transgene was assessed. RESULTS: All surviving fetuses that had been injected in the liver (8/9 fetuses), peritoneum (3/3 fetuses), or lung (9/10 fetuses) showed a signal on bioluminescence imaging scan up to 30 weeks. None of the survivors displayed seroconversion against the transgene. CONCLUSION: In the rat model, the administration of lentiviral vectors into the fetal lung and liver resulted in long-term transgene expression without detectable humoral immune response.

引用

页码：352.e1 / 352.e6

页数：6

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