Structural analysis and biomedical applications of dextran produced by a new isolate Pediococcus pentosaceus screened from biodiversity hot spot Assam

被引：34

作者：

Patel, Seema ^{[1
]}

Kasoju, Naresh ^{[1
]}

Bora, Utpal ^{[1
]}

Goyal, Arun ^{[1
]}

机构：

[1] Indian Inst Technol Guwahati, Dept Biotechnol, Gauhati 781039, Assam, India

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 17期

关键词：

Pediococcus pentosaceus; Dextransucrase; Dextran; Rheometry; HeLa cells; LEUCONOSTOC-DEXTRANICUM; IDENTIFICATION; HYDROGELS; GLUCAN;

D O I：

10.1016/j.biortech.2010.03.063

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Dextran produced by a natural isolate of Pediococcus pentosaceus, screened from Assam, in the Northeastern region of India, was estimated, purified, structure characterised and functionality analysed. The dextran concentration in the cell free supernatant of the isolate P. pentosaceus was 10.2 mg/ml. FT-IR analysis revealed the hydroxyl and carboxyl functional groups present in the dextran. H-1 NMR and C-13 NMR spectral data revealed that the dextran has a linear backbone of alpha-(1 -> 6) linked D-glucose residues. The decrease in viscosity of dextran solution with the increase in shear rate, threw light on its typical non-Newtonian pseudoplastic behaviour. The cytotoxicity tests on human cervical cancer (HeLa) cell line was studied which showed the dextran is non-toxic and biocompatible, rendering it safe for drug delivery, tissue engineering and various other biomedical applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：6852 / 6855

页数：4