Gaussian beam in two-photon fluorescence imaging of rat brain microvessel

被引：10

作者：

Shi, Lingyan ^{[1
,2
,3
,4
,5
]}

Rodriguez-Contreras, Adrian ^{[2
,3
,4
,5
]}

Alfano, Robert R. ^{[3
,4
,5
]}

机构：

[1] CUNY City Coll, Dept Biomed Engn, New York, NY 10031 USA

[2] CUNY City Coll, Dept Biol, New York, NY 10031 USA

[3] CUNY City Coll, Inst Ultrafast Spect & Lasers, New York, NY 10031 USA

[4] CUNY City Coll, Dept Elect Engn, New York, NY 10031 USA

[5] CUNY City Coll, Dept Phys, New York, NY 10031 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2014年 / 19卷 / 12期

关键词：

Gaussian beam; two-photon microscopy; spot size; depth of focus; rat brain; in vivo; BESSEL; MICROSCOPY;

D O I：

10.1117/1.JBO.19.12.126006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：5

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