Determination of Microcystins in Aquatic Environment with High Performance Liquid Chromatography

被引：6

作者：

Liang Li-Li ^{[1
]}

Gong Ai-Jun ^{[1
]}

Li Hong-Mei ^{[2
]}

Cao Yan-Qiu ^{[1
]}

Li Bao-Qin ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Beijing 100083, Peoples R China

[2] China Natl Measuring Sci Res Inst, Beijing 100013, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2010年 / 38卷 / 05期

关键词：

Microcystins; High performance liquid chromatography; MASS-SPECTROMETRY; WATER; LR; COLUMN;

D O I：

10.3724/SP.J.1096.2010.00740

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The determination method for microcystins (MCs) in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V(0.1% trifluoroacetic acid): V( methanol) = 42:58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 mu g/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.

引用

页码：740 / 742

页数：3

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