A taxonomic revision to Portulaca (Portulacaceae) in Cuba is presented. Fourteen species are recognized: 11 native (one endemic), one naturalized, and two cultivated. Portulaca avinoamii Garcia-Beltran is proposed as a new name for P. tuberculata (Danin & H.G. Baker) Danin (non P. tuberculata Leon). The lectotypes for 21 names of Portulaca are designated: P. biloba, P. brevifolia, P. cubensis, P. elatior, P. erecta, P. gagatosperma, P. grandiflora, P. halimoides var. brevipilosa, P. martinicensis, P. microphylla, P. nana, P. paucistaminata, P. phaeosperma, P. poliosperma, P. poliosperma var. bistigmatata, P. poliosperma var. cubensis, P. poliosperma var. minor, P. procumbens, P. rubricaulis, P. teretifolia and P. umbraticola. The second step lectotype of P. coronata is designated. Four new synonyms for P. biloba are presented: Portulaca poliosperma var. cubensis, P. poliosperma var. tristigmatata, P. poliosperma var. bistigmatata and P. cinerea, and one new synonym for P. pilosa is proposed: Portulaca halimoides var. brevipilosa. An identification key for all species is presented, and updated descriptions, nomenclatural information, geographic distributions, citation of all specimens examined, and habitat descriptions for all the species are included here.