Use of antibiotic disks to evolve drug-resistant bacteria

被引:2
|
作者
Flanagan, J. Nicole [1 ]
Steck, Todd R. [1 ]
机构
[1] Univ North Carolina Charlotte, Dept Biol Sci, Charlotte, NC 28223 USA
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2018年 / 111卷 / 09期
基金
美国国家卫生研究院;
关键词
Antibiotic resistance selection; Burkholderia multivorans; SENSITIVITY; EVOLUTION;
D O I
10.1007/s10482-018-1055-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The methods used to generate antibiotic-resistant bacterial strains can be labour-intensive, costly, lengthy and/or prone to plate-to-plate variation. We propose a simple, inexpensive and easily replicated method to expose bacteria to a continuous gradient of antibiotic concentration, providing an environment of positive selective pressure for evolution of antibiotic-resistant strains.
引用
收藏
页码:1719 / 1722
页数:4
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