Comparison of the Quality of Protein Crystals Grown by CLPC Seeds Method

被引：1

作者：

Li, Jin ^{[1
,2
]}

Yan, Er-Kai ^{[1
]}

Liu, Yue ^{[1
]}

Wu, Zi-Qing ^{[1
]}

Liu, Ya-Li ^{[1
]}

Hou, Hai ^{[1
]}

Zhang, Chen-Yan ^{[1
]}

Lu, Qin-Qin ^{[1
]}

Deng, Xu-Dong ^{[1
]}

Yin, Da-Chuan ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Inst Special Environm Biophys, Key Lab Space Biosci & Space Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China

[2] Shaan Xi Univ Chinese Med, Coll Pharm, Xian 712046, Shanxi, Peoples R China

来源：

CRYSTALS | 2019年 / 9卷 / 10期

基金：

中国国家自然科学基金;

关键词：

CLPC seeds; crystal quality; resolution; mosaicity; LINKED ENZYME CRYSTALS; X-RAY; CROSS-LINKING; CRYSTALLIZATION; LIGAND; B7-H6;

D O I：

10.3390/cryst9100501

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.

引用

页数：12

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