Epitope-blocking enzyme-linked immunosorbent assays for detection of West Nile virus antibodies in domestic mammals

被引:91
|
作者
Blitvich, BJ
Bowen, RA
Marlenee, NL
Hall, RA
Bunning, ML
Beaty, BJ [1 ]
机构
[1] Colorado State Univ, Arthropod Borne & Infect Dis Lab, Dept Microbiol Immunol & Parasitol, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Anim Reprod & Biotechnol Lab, Equine Ctr, Ft Collins, CO 80523 USA
[3] Univ Queensland, Dept Microbiol & Parasitol, St Lucia, Qld 4072, Australia
[4] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Ft Collins, CO 80522 USA
[5] USAF, Off Surg Gen, Washington, DC 20332 USA
关键词
D O I
10.1128/JCM.41.6.2676-2679.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
引用
收藏
页码:2676 / 2679
页数:4
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