Analysis of the mechanical properties of chromosomes in air and liquid by AFM

被引：1
|
作者：
Wang, Bowei [1 ,2 ]
Yang, Fan [1 ,2 ]
Dong, Jianjun [1 ,2 ]
Li, Jiani [1 ,2 ]
Wang, Ying [1 ,2 ]
Qu, Kaige [1 ,2 ]
Wei, Huimiao [3 ]
Wang, Zuobin [1 ,2 ,4 ,5 ]
机构：
[1] Changchun Univ Sci & Technol, Key Lab Cross Scale Micro & Nano Mfg, Minist Educ, Changchun 130022, Peoples R China
[2] Changchun Univ Sci & Technol, Int Res Ctr Nano Handling & Mfg China, Changchun 130022, Peoples R China
[3] Changli Nano Biotechnol Ltd, Changchun 130022, Peoples R China
[4] Univ Bedfordshire, JR3CN, Luton LU1 3JU, Beds, England
[5] Univ Bedfordshire, IRAC, Luton LU1 3JU, Beds, England
来源：
2021 IEEE INTERNATIONAL CONFERENCE ON MANIPULATION, MANUFACTURING AND MEASUREMENT ON THE NANOSCALE (3M-NANO) | 2021年
基金：
国家重点研发计划;
关键词：
atomic force microscopy; chromosome; morphology; mechanical properties; ATOMIC-FORCE MICROSCOPY; CHROMATIN;
D O I：
10.1109/3M-NANO49087.2021.9599772
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Chromosomes contain all the genomic information thus making the study of chromosomes practical and significant. Herein, the morphologies of chromosomes prepared with different methods were monitored by atomic force microscopy (AFM). Moreover, the mechanical properties of chromosomes in air and liquid were investigated quantitatively through AFM-based force spectroscopy. The differences of chromosome morphologies and mechanical properties caused by sample preparations indicated that the chromosome characterization in liquid was more meaningful to rep cal the physiological characteristics of chromosomes.
引用
收藏
页码：43 / 46
页数：4
相关论文
共 50 条
  • [1] Analysis of the effect of mechanical properties of liquid and geometrical parameters of cantilever on the frequency response function of AFM
    Korayem, Moharam Habibnejad
    Sharahi, H. Jiryaei
    INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY, 2011, 57 (5-8): : 477 - 489
  • [2] Analysis of the effect of mechanical properties of liquid and geometrical parameters of cantilever on the frequency response function of AFM
    Moharam Habibnejad Korayem
    H. Jiryaei Sharahi
    The International Journal of Advanced Manufacturing Technology, 2011, 57 : 477 - 489
  • [3] High-speed AFM of human chromosomes in liquid
    Picco, L. M.
    Dunton, P. G.
    Ulcinas, A.
    Engledew, D. J.
    Hoshi, O.
    Ushiki, T.
    Miles, M. J.
    NANOTECHNOLOGY, 2008, 19 (38)
  • [4] AFM Investigation of Mechanical Properties of Dentin
    Cohen, Sidney R.
    Apter, Nathan
    Jesse, Stephen
    Kalinin, Sergei
    Barlam, David
    Peretz, Adi Idit
    Ziskind, Daniel
    Wagner, H. Daniel
    ISRAEL JOURNAL OF CHEMISTRY, 2008, 48 (02) : 65 - 72
  • [5] NANOSCALE MECHANICAL PROPERTIES OF SINGLE BIOMOLECULES BY AFM
    Schon, Peter
    Gosa, Maria
    Vancso, G. Julius
    REVUE ROUMAINE DE CHIMIE, 2013, 58 (7-8) : 577 - 583
  • [6] The effect of the temperature of liquid air on the mechanical properties of iron and its alloys
    Dewar, J
    Hadfield, RA
    ANNALES DE CHIMIE ET DE PHYSIQUE, 1905, 4 : 556 - 574
  • [7] Imaging and force measurement of LDL and HDL by AFM in air and liquid
    Gan, Chaoye
    Ao, Meiying
    Liu, Zhanghua
    Chen, Yong
    FEBS OPEN BIO, 2015, 5 : 276 - 282
  • [8] AFM imaging and analysis of local mechanical properties for detection of surface pattern of functional groups
    Knotek, Petr
    Chanova, Eliska
    Rypacek, Frantisek
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2013, 33 (04): : 1963 - 1968
  • [9] Nanoscale mechanical properties of chitosan hydrogels as revealed by AFM
    Ben Bouali, A.
    Montembault, A.
    David, L.
    Von Boxberg, Y.
    Viallon, M.
    Hamdi, B.
    Nothias, F.
    Fodil, R.
    Fereol, S.
    PROGRESS IN BIOMATERIALS, 2020, 9 (04) : 187 - 201
  • [10] Loops Determine the Mechanical Properties of Mitotic Chromosomes
    Zhang, Yang
    Heermann, Dieter W.
    PLOS ONE, 2011, 6 (12):
12345