Geroprotective potential of in vitro bioactive compounds isolated from yarrow (Achilleae millefolii L.) cell cultures

Introduction. There is an urgent need for geroprotectors that prevent premature aging, especially antioxidants of plant origin. Due to the shortage of medicinal plant materials, scientists look for alternative sources of bioactive compounds of phenolic nature, for example, cell cultures and organs of higher plants. This paper describes a study of the geroprotective potential of in vitro bioactive compounds isolated from yarrow (Achilleae millefolii L.) cell cultures. Study objects and methods. Callus, suspension and hairy root cultures of A. millefolii were obtained by in vitro cultivation on modified nutrient media. High performance liquid chromatography (HPLC) was used to analyze the composition of the cell cultures and ethanol extracts. The extracts' antimicrobial activity was studied by the disk diffusion method and their antioxidant activity was measured based on titration of a potassium permanganate solution. Results and discussion. The biomass of all yarrow cell cultures contained essential oils, flavonoids, glycosides, phenolic acids, carotenoids, as well as vitamins C and E. The suspension culture had a higher content of essential oils, flavonoids and glycosides than the callus and hairy root cultures. The extracts of the A. millefolii suspension culture also contained geroprotectors - phenylpropanoids, flavonoids, and simple phenols, with a prevalence of caffeic acid, cynaroside, 4,5-dicofeylquinic acid, apigenin, and luteolin. In addition, HPLC revealed the presence of cumic aldehyde, umbelliferone, 3-caffeylquinic acid, and caffeic acid - the bioactive compounds previously not reported in yarrow. In vitro experiments with the extracts proved their antimicrobial and antioxidant activity. Conclusion. The complex of bioactive compounds isolated from the biomass of yarrow suspension culture provides this plant with potential geroprotective properties. Thus, yarrow can be used to create nutraceuticals that prevent premature aging.