Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid

被引：29
|
作者：
Alimolaei, Mojtaba [1 ,2 ]
Golchin, Mehdi [1 ]
Daneshvar, Hamid [3 ]
机构：
[1] Shahid Bahonar Univ Kerman, Dept Pathobiol, Fac Vet Med, Kerman, Iran
[2] Razi Vaccine & Serum Res Inst, Dept Anaerob Bacterial Vaccine Res & Prod, Kerman Branch, Kerman, Iran
[3] Kerman Univ, Dept Immunol, Sch Med, Kerman, Iran
来源：
INFECTION GENETICS AND EVOLUTION | 2016年 / 40卷
基金：
美国国家科学基金会;
关键词：
Clostridium perfringens; Lactobacillus casei; Vaccine; Toxoid; Enterotoxemia; LACTIC-ACID BACTERIA; NECROTIC ENTERITIS; NEUTRALIZING ANTIBODIES; NASAL IMMUNIZATION; PROTECTS MICE; IMMUNE-SYSTEM; TETANUS TOXIN; ALPHA-TOXIN; MUCOSAL; INDUCTION;
D O I：
10.1016/j.meegid.2016.03.020
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by epsilon-toxin. A mutated epsilon-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-epsilon recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated epsilon-toxin. This study showed that the LC-pT1NX-epsilon could be a promising vaccine candidate against the enterotoxemia disease. (c) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：282 / 287
页数：6
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