Acquired acid adaptation of Listeria monocytogenes during its planktonic growth enhances subsequent survival of its sessile population to disinfection with natural organic compounds

The abilities of Listeria monocytogenes to adapt to stress and also to attach to surfaces are believed to contribute to its persistence in food processing environments. In this study, the possible influence of acid adaptation of L. monocytogenes cells during their planktonic growth on their subsequent resistance against 3 different natural disinfection treatments (0.05% lactic acid, 1% essential oil or 100% hydrosol of S. thymbra) upon their attachment to stainless steel (SS) was evaluated by simultaneously using the bead vortexing technique and a conductance method. Prior to disinfection, both nonadapted and acid-adapted stationary phase bacteria were left to attach to SS coupons statically incubated for 10 days into Brain Heart Infusion (BHI) broth under 3 different temperatures (5,16 or 30 degrees C). Results revealed that both essential oil and hydrosol presented sufficient bactericidal activity against all formed sessile populations, always resulting in counts following disinfection below the plate counting detection limit. However, the conductance method, able to detect metabolically active sessile bacteria unable to be recovered by the bead vortexing, revealed the positive influence of previously acquired acid adaptation on disinfection resistance of attached cells against these plant extracts. A similar effect of acid adaptation was also evident for lactic acid disinfection. (C) 2014 Elsevier Ltd. All rights reserved.