Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

被引：173
|
作者：
Gimeno, E [1 ]
Castellote, AI [1 ]
Lamuela-Raventós, RM [1 ]
de la Torre, MC [1 ]
López-Sabater, MC [1 ]
机构：
[1] Univ Barcelona, Fac Farm, Ctr Referencia Tecnol Aliments, CeRTA,Dpt Nutr Bromatol, E-08028 Barcelona, Spain
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 881卷 / 1-2期
关键词：
oils; food analysis; vitamins; tocopherols;
D O I：
10.1016/S0021-9673(00)00219-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (ol-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%). (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：251 / 254
页数：4
相关论文
共 50 条
  • [1] Rapid determination by reversed-phase high-performance liquid chromatography of Vitamins A and E in infant formulas
    Mendoza, BR
    Pons, SM
    Bargalló, AIC
    López-Sabater, MC
    JOURNAL OF CHROMATOGRAPHY A, 2003, 1018 (02) : 197 - 202
  • [2] Determination of vitamin E acid succinate in biodegradable microspheres by reversed-phase high-performance liquid chromatography
    Sancho, CM
    Vanrell, RH
    Negro, S
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2004, 42 (01) : 43 - 48
  • [3] Rapid determination of mycophenolic acid in plasma by reversed-phase high-performance liquid chromatography
    Pastore, A
    Lo Russo, A
    Piemonte, F
    Mannucci, L
    Federici, G
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 776 (02): : 251 - 254
  • [4] Rapid determination of nitrite by reversed-phase high-performance liquid chromatography with fluorescence detection
    Li, H
    Meininger, CJ
    Wu, GY
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 746 (02): : 199 - 207
  • [5] RAPID SIMULTANEOUS DETERMINATION FOR CATECHOLAMINES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    PAPADOYANNIS, I
    ARZOGLOU, P
    ASVESTA, S
    GRAMMATOPOULOS, D
    LAZARIDIS, A
    ANALYTICAL LETTERS, 1989, 22 (03) : 545 - 554
  • [6] Rapid assay of vitamin E in vegetable oils by reversed-phase capillary electrochromatography
    Aturki, Z
    D'Orazio, G
    Fanali, S
    ELECTROPHORESIS, 2005, 26 (4-5) : 798 - 803
  • [7] Determination of linezolid in plasma by reversed-phase high-performance liquid chromatography
    Peng, GW
    Stryd, RP
    Murata, S
    Igarashi, M
    Chiba, K
    Aoyama, H
    Aoyama, M
    Zenki, T
    Ozawa, N
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 20 (1-2) : 65 - 73
  • [8] Analysis of triacylglyceride hydroperoxides in vegetable oils by nonaqueous reversed-phase high-performance liquid chromatography with ultraviolet detection
    Bauer-Plank, C
    Steenhorst-Slikkerveer, L
    JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2000, 77 (05) : 477 - 482
  • [9] DETERMINATION OF URANIUM BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    CASOLI, A
    MANGIA, A
    PREDIERI, G
    ANALYTICAL CHEMISTRY, 1985, 57 (02) : 561 - 563
  • [10] Simple and rapid determination of nevirapine in human serum by reversed-phase high-performance liquid chromatography
    Lopez, RM
    Pou, L
    Gomez, MR
    Ruiz, I
    Monterde, J
    JOURNAL OF CHROMATOGRAPHY B, 2001, 751 (02): : 371 - 376
12345