Collagen immobilized PVA hydrogel-hydroxyapatite composites prepared by kneading methods as a material for peripheral cuff of artificial cornea

被引：65

作者：

Kobayashi, H

Kato, M

Taguchi, T

Ikoma, T

Miyashita, H

Shimmura, S

Tsubota, K

Tanaka, J

机构：

[1] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050044, Japan

[2] Tokyo Dent Coll, Dept Ophthalmol, Chiba, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2004年 / 24卷 / 6-8期

关键词：

artificial cornea; peripheral cuff; PVA-collagen-hydroxyapatite composites hydrogel;

D O I：

10.1016/j.msec.2004.08.038

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In order to achieve the firm fixation of the artificial cornea to host tissues, composites of collagen-immobilized poly(vinyl alcohol) hydrogel with hydroxyapatite were synthesized by a hydroxyapatite particles kneading method. The preparation method, characterization, and the results of corneal cell adhesion and proliferation on the composite material were studied. PVA-COL-HAp composites were successfully synthesized. A micro-porous structure of the PVA-COL-HAp could be introduced by hydrochloric acid treatment and the porosity could be controlled by the pH of the hydrochloric acid solution, the treatment time, and the crystallinity of the HAp particles. Chick embryonic keratocyto-like cells were well attached and proliferated on the PVA-COL-HAp composites. This material showed potential for keratoprosthesis application. Further study such as a long-term animal study is now required. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：729 / 735

页数：7