Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis

被引：12
|
作者：
Narayanan, S [1 ]
Selvakumar, S [1 ]
Aarati, R [1 ]
Vasan, SK [1 ]
Narayanan, PR [1 ]
机构：
[1] TB Res Ctr, Dept Immunol, Chetput 600031, Chennai, India
来源：
FEMS MICROBIOLOGY LETTERS | 2000年 / 192卷 / 02期
关键词：
acetamidase; gene regulation; transcription start site; promoter; Mycobacterium smegmatis;
D O I：
10.1016/S0378-1097(00)00447-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：263 / 268
页数：6
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