DNA binding and cleavage studies of copper(II) complex containing N2O2 Schiff base ligand

A new series of copper (II) complexes [Cu(L1-5)](ClO4)] [1-5] were synthesized by condensation of salicylaldehyde with various diamines (1,2-diamino ethane, 1,3-diamino propane, 1,2-diamino benzene, 2-amino-benzyl amine and 1,8-diamino naphthalene) and characterized by several analytical techniques. The precursor ligand structure was confirmed by single crystal X-ray diffraction (XRD) techniques. The viscosity measurement and molecular docking studies were also carried out for newly synthesized Cu(II) complexes. Cyclic voltametry (CV) study showed a quasi-reversible reduction wave in cathodic region confirm the presence of Cu2+. From the electronic spectral and DNA binding studies (K-b values are within the range from 0.47 x 10(4) M-1 to 1.18 x 10(5) M-1) it evidences that Cu(II) complexes were intercalated with DNA, this observation was further confirmed by fluorescence measurement studies (K-app similar to 0.22 x 10(5) M-1 -0.26 x 10(6) M-1). Comparatively the maximum DNA cleavage was observed in the complex (5) and the possible mechanism of DNA cleavage activity has also been discussed. In addition, the interaction of complexes (1-5) with bovine serum albumin (BSA) was also examined. These encouraging results may pave the way towards the development of novel therapeutic agents in cancer biology.