siFLIMIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

被引：0
|
作者：
Raspe, Marcel [1 ]
Kedziora, Katarzyna M. [1 ]
van den Broek, Bram [1 ]
Zhao, Qiaole [2 ]
de Jong, Sander [3 ]
Herz, Johan [3 ]
Mastop, Marieke [4 ]
Goedhart, Joachim [4 ,5 ]
Gadella, Theodorus W. J. [4 ,5 ]
Young, Ian T. [2 ]
Jalink, Kees [1 ,4 ,5 ]
机构：
[1] Netherlands Canc Inst, Dept Cell Biol, Amsterdam, Netherlands
[2] Delft Univ Technol, Dept Imaging Phys, Delft, Netherlands
[3] Lambert Instruments BV, Groningen, Netherlands
[4] Univ Amsterdam, Sect Mol Cytol, Swammerdam Inst Life Sci, Amsterdam, Netherlands
[5] Van Leeuwenhoek Ctr Adv Microscopy, Amsterdam, Netherlands
来源：
NATURE METHODS | 2016年 / 13卷 / 06期
基金：
欧洲研究理事会;
关键词：
TIME-RESOLVED FLUORESCENCE; MICROSCOPY; COMPLEXES; CARCINOMA; HISTAMINE; CELLS;
D O I：
10.1038/NMETH.3836
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
引用
收藏
页码：501 / +
页数：6
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