Molecular characterization of a novel RhoGAP, RRC-1 of the nematode Caenorhabditis elegans

被引：2
|
作者：
Delawary, Mina
Nakazawa, Takanobu
Tezuka, Tohru
Sawa, Mariko
Iino, Yuichi
Takenawa, Tadaomi
Yamamoto, Tadashi
机构：
[1] Univ Tokyo, Div Oncol, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan
[2] Univ Tokyo, Inst Med Sci, Div Biochem, Tokyo, Japan
[3] Univ Tokyo, Grad Sch Sci, Mol Genet Res Lab, Tokyo, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 02期
基金：
美国国家卫生研究院;
关键词：
C; elegans; RhoGAP; RRC-1; p250GAP; TCGAP;
D O I：
10.1016/j.bbrc.2007.03.192
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The GTPase-activating proteins for Rho family GTPases (RhoGAP) transduce diverse intracellular signals by negatively regulating Rho family GTPase-mediated pathways. In this study, we have cloned and characterized a novel RhoGAP for Rac1 and Cdc42, termed RRC-1, from Caenorhabditis elegans. RRC-1 was highly homologous to mammalian p250GAP and promoted GTP hydrolysis of Rac1 and Cdc42 in cells. The rrc-1 mRNA was expressed in all life stages. Using an RRC-1::GFP fusion protein, we found that RRC-1 was localized to the coelomocytes, excretory cell, GLR cells, and uterine-seam cell in adult worms. These data contribute toward understanding the roles of Rho family GTPases in C elegans. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：377 / 382
页数：6
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