Electrospray and APCI analysis of polyhydroxyalkaloids using positive and negative collision induced dissociation experiments in a quadrupole ion trap

被引：15
|
作者：
Egan, MJ [1 ]
Kite, GC
Porter, EA
Simmonds, MSJ
Howells, S
机构：
[1] Royal Bot Gardens, Jodrell Lab, Kew TW9 3AB, Surrey, England
[2] Univ Greenwich, London SE18 6PF, England
来源：
ANALYST | 2000年 / 125卷 / 08期
关键词：
D O I：
10.1039/b003159n
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Atmospheric pressure chemical ionisation (APCI) and electrospray (ES) are compared as ion sources in the analysis of polyhydroxyalkaloids (PHAs) by liquid chromatography mass spectrometry (LC-MS) and collision induced dissociation (CID) product ion spectra, from tandem mass spectrometry (MS-MS) experiments in a quadrupole ion trap, are reported for 12 naturally occurring PHAs. APCI was found to be a more useful source than ES, as APCI could be used to generate deprotonated molecule ions in negative mode and for some isomeric PHAs the negative CID product ion spectra were more diagnostic than the positive product ion spectra. On-column detection limits were also approximately 32 times lower by positive APCI than ES. The work provides data that will facilitate screening and characterisation of this group of important natural products in plant and fungal extracts.
引用
收藏
页码：1409 / 1414
页数：6
相关论文
共 50 条
  • [1] On the detailed mechanisms of collision-induced dissociation experiments performed by electrospray ion trap
    Catinella, S
    Pelizzi, N
    Barboso, S
    Favretto, D
    Seraglia, R
    Traldi, P
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (20) : 1897 - 1902
  • [2] Collision-induced dissociation of a series of coumarins studied by positive- and negative-ion electrospray triple quadrupole tandem mass spectrometry
    Lerman, L
    Klonkay, AC
    Filipovic, A
    Elenkov, I
    EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2004, 10 (04) : 523 - 539
  • [3] Collision-induced dissociation of glycero phospholipids using electrospray ion-trap mass spectrometry
    Larsen, Å
    Uran, S
    Jacobsen, PB
    Skotland, T
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (24) : 2393 - 2398
  • [4] Dynamic collision-induced dissociation of peptides in a quadrupole ion trap mass spectrometer
    Collin, Olivier L.
    Beier, Matthias
    Jackson, Glen P.
    ANALYTICAL CHEMISTRY, 2007, 79 (14) : 5468 - 5473
  • [5] Thermally assisted collision-induced dissociation in a quadrupole ion trap mass spectrometer
    Racine, Alawee H.
    Payne, Anne H.
    Remes, Philip M.
    Glish, Gary L.
    ANALYTICAL CHEMISTRY, 2006, 78 (13) : 4609 - 4614
  • [6] ELECTROSPRAY IONIZATION OF PORPHYRINS USING A QUADRUPOLE ION TRAP FOR MASS ANALYSIS
    VANBERKEL, GJ
    MCLUCKEY, SA
    GLISH, GL
    ANALYTICAL CHEMISTRY, 1991, 63 (11) : 1098 - 1109
  • [7] DESIREE electrospray ion source test bench and setup for collision induced dissociation experiments
    de Ruette, N.
    Wolf, M.
    Giacomozzi, L.
    Alexander, J. D.
    Gatchell, M.
    Stockett, M. H.
    Haag, N.
    Zettergren, H.
    Schmidt, H. T.
    Cederquist, H.
    REVIEW OF SCIENTIFIC INSTRUMENTS, 2018, 89 (07):
  • [8] Sulfate migration in oligosaccharides induced by negative ion mode ion trap collision-induced dissociation
    Kenny, Diarmuid T.
    Issa, Samah M. A.
    Karlsson, Niclas G.
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (18) : 2611 - 2618
  • [9] Mass spectrometry of stanozolol and its analogues using electrospray ionization and collision-induced dissociation with quadrupole-linear ion trap and linear ion trap-orbitrap hybrid mass analyzers
    Thevis, M
    Makarov, AA
    Horning, S
    Schänzer, W
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (22) : 3369 - 3378
  • [10] Study of mechanisms involved in the collision-induced dissociation of carboxylate anions from glycerophospholipids using negative ion electrospray tandem quadrupole mass spectrometry
    Hvattum, E
    Hagelin, G
    Larsen, A
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1998, 12 (19) : 1405 - 1409
12345