Molecular characterisation of Staphylococcus aureus from some artisanal Brazilian dairies

被引：27
|
作者：
Alves, Virginia Farias [1 ]
Nino-Arias, Fabian Camilo [2 ]
Pitondo-Silva, Andre [2 ]
Frazilio, Diego de Araujo [2 ]
Goncalves, Larissa de Oliveira [1 ]
Toubas, Luiza Chaul [1 ]
Sapateiro Torres, Ieda Maria [1 ]
Oxaran, Virginie [3 ]
Dittmann, Karen Kiesbye [3 ]
Pereira De Martinis, Elaine Cristina [2 ]
机构：
[1] Univ Fed Goias, Fac Farm, Goiania, Go, Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, Brazil
[3] Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby, Denmark
来源：
INTERNATIONAL DAIRY JOURNAL | 2018年 / 85卷
基金：
巴西圣保罗研究基金会; 英国惠康基金;
关键词：
MINAS FRESCAL CHEESE; FIELD GEL-ELECTROPHORESIS; METHICILLIN-RESISTANT; SAO-PAULO; LISTERIA-MONOCYTOGENES; ANTIBIOTIC-RESISTANCE; BOVINE-MILK; FOOD; DIVERSITY; FARMS;
D O I：
10.1016/j.idairyj.2018.06.008
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The clonal relationships and antimicrobial resistance of Staphylococcus aureus isolates from three fresh Minas cheese production lines were investigated. Putative S. aureus were confirmed by 16S rRNA gene sequencing and were characterised by multilocus sequence typing (MLST) and antimicrobial susceptibility. Overall, 33 out of 64 samples were contaminated with S. aureus (51.6%). The contamination rates of the dairies were 60.8%, 21% and 68.2%. Thirty representative isolates were selected to be typed by MLST and five sequence types (ST) were found (ST1, 97, 126, 3531), including the new ST 3816. The clonal complex 1 (CC1) was dominant. Resistance to commonly used antimicrobials was observed but only one strain was resistant to methicillin. This methicillin resistant S. aureus strain belonged to the CC126 that is commonly associated with mastitis in ruminants. The dissemination of zoonotic bacteria through the food chain is not a frequent event but represents a concern to public health. (C) 2018 Published by Elsevier Ltd.
引用
收藏
页码：247 / 253
页数：7
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