Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings

被引:80
|
作者
Balotf, Sadegh [1 ]
Kavoosi, Gholamreza [1 ]
Kholdebarin, Bahman [2 ]
机构
[1] Shiraz Univ, Inst Biotechnol, Shiraz 7144165186, Iran
[2] Shiraz Univ, Fac Sci, Dept Biol, Shiraz 7144165186, Iran
基金
美国国家科学基金会;
关键词
wheat seedlings; nitrate reductase; nitrite reductase; glutamine synthetase; glutamate synthase; ARABIDOPSIS-THALIANA; AMINO-ACIDS; POSTTRANSLATIONAL REGULATION; AMMONIUM TOXICITY; HIGHER-PLANTS; NO3-UPTAKE; ASSIMILATION; LIGHT; ACCUMULATION; LEAVES;
D O I
10.1002/bab.1362
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of this study was to examine the expression and activity of nitrate reductase (NR, EC 1.7.1.1), nitrite reductase (NiR, EC 1.7.2.2), glutamine synthetase (GS, EC 6.3.1.2), and glutamate synthase (GOGAT, EC 1.4.7.1) in response to potassium nitrate, ammonium chloride, and ammonium nitrate in nitrogen-starved wheat seedlings. Plants were grown in standard nutrient solution for 17 days and then subjected to nitrogen starvation for 7 days. The starved plants were supplied with potassium nitrate ammonium nitrate and ammonium chloride (50mM) for 4 days and the leaves were harvested. The relative expression of NR, NiR, GS, and GOGAT as well as the enzyme activities were investigated. Nitrogen starvation caused a significant decrease both in transcript levels and in NR, NiR, GS, and GOGAT activities. Potassium nitrate and ammonium nitrate treatments restored NR, NiR, GS, and GOGAT expressions and activities. Ammonium chloride increased only the expressions and activities of GS and GOGAT in a dose-dependent manner. The results of our study highlight the differential effects between the type and the amount of nitrogen salts on NR, NiR, GS, and GOGAT activities in wheat seedlings while potassium nitrate being more effective.
引用
收藏
页码:220 / 229
页数:10
相关论文
共 50 条