Because of the great interest in Y-chromosome polymorphisins not only in the forensic field but also in evolutionary studies, the number of described Y-chromosome markers has been increased considerably in the recent literature. During the last few years, new Y-chromosome polymorphisins have been described, including binary polymorphisms, microsatellites and minisatellites. In this study, eight recently described tetranucleotide microsatellites have been analysed: DYS434, DYS437, DYS439, Y-GATA A7.1, Y-GATA A7.2, Y-GATA A.10, Y-GATA C4 and Y-GATA H4. Gene and haplotype frequencies have been estimated in the Valencian population, to determine highly informative haplotypes, using these new Y-STRs combined with the classical ones. (C) 2003 Elsevier Science B.V. All rights reserved.