Effect of Amphotericin B antibiotic on the properties of model lipid membrane

被引:5
|
作者
Kiryakova, S. [1 ]
Dencheva-Zarkova, M. [1 ]
Genova, J. [1 ]
机构
[1] Bulgarian Acad Sci, Inst Solid State Phys, Sofia 1784, Bulgaria
关键词
CHOLESTEROL;
D O I
10.1088/1742-6596/558/1/012027
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Model membranes formed from natural and synthetic lipids are an interesting object for scientific investigations due to their similarity to biological cell membrane and their simple structure with controlled composition and properties. Amphotericin B is an important polyene antifimgal antibiotic, used for treatment of systemic fungal infections. It is known from the literature that the studied antibiotic has a substantial effect on the transmembrane ionic channel structures. When applied to the lipid membranes it has the tendency to create pores and in this way to affect the structure and the properties of the membrane lipid bilayer. In this work the thermally induced shape fluctuations of giant quasi-spherical Liposomes have been used to study the influence of polyene antibiotic amphotericin B on the elastic properties of model lipid membranes, it have been shown experimentally that the presence of 3 inol % of AmB in the lipid membrane reduces the bending elasticity of the lipid membrane for both studied cases: pure SOPC membrane and mixed SOPC-Cholesterol membrane. Interaction of the amphotericin B with bilayer lipid membranes containing channels have been studied in this work. Model membranes were self-assembled using the patch-clamp and tip-dip patch clamp technique. We have found that amphotericin B is an ionophore and reduces the resistance of the lipid bilayer.
引用
收藏
页数:6
相关论文
共 50 条
  • [1] An effect of antibiotic amphotericin B on ion transport across model lipid membranes and tonoplast membranes
    Herec, M
    Dziubinska, H
    Trebacz, K
    Morzycki, JW
    Gruszecki, WI
    BIOCHEMICAL PHARMACOLOGY, 2005, 70 (05) : 668 - 675
  • [2] EFFECT OF AMPHOTERICIN-B AND FILIPIN ON MEMBRANE LIPID POLYMORPHISM
    ELLENA, JF
    HILL, BD
    MCGARVEY, GJ
    CAFISO, DS
    BIOPHYSICAL JOURNAL, 1987, 51 (02) : A529 - A529
  • [3] Effect of antibiotic amphotericin B on structural and dynamic properties of lipid membranes formed with egg yolk phosphatidylcholine
    Herec, Monika
    Islamov, Akhmed
    Kuklin, Alexander
    Gagos, Mariusz
    Gruszecki, Wieslaw I.
    CHEMISTRY AND PHYSICS OF LIPIDS, 2007, 147 (02) : 78 - 86
  • [4] Organization of antibiotic amphotericin B in model lipid membranes. A mini review
    Gruszecki, WI
    Gagos, M
    Herec, M
    Kernen, P
    CELLULAR & MOLECULAR BIOLOGY LETTERS, 2003, 8 (01) : 161 - 170
  • [5] PROPERTIES OF AMPHOTERICIN-B CHANNELS IN A LIPID BILAYER
    ERMISHKIN, LN
    KASUMOV, KM
    POTSELUYEV, VM
    BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 470 (03) : 357 - 367
  • [6] Lipid Membrane-Binding Properties of Amphotericin B Deoxycholate (Fungizone) Using Surface Plasmon Resonance
    Oka, Masako
    Kamimori, Hiroshi
    ANALYTICAL SCIENCES, 2013, 29 (07) : 697 - 702
  • [7] Lipid Membrane-Binding Properties of Amphotericin B Deoxycholate (Fungizone) Using Surface Plasmon Resonance
    Masako Oka
    Hiroshi Kamimori
    Analytical Sciences, 2013, 29 : 697 - 702
  • [8] Pharmacokinetics and Pharmacodynamics of Amphotericin B Deoxycholate, Liposomal Amphotericin B, and Amphotericin B Lipid Complex in an In Vitro Model of Invasive Pulmonary Aspergillosis
    Lestner, Jodi M.
    Howard, Susan J.
    Goodwin, Joanne
    Gregson, Lea
    Majithiya, Jayesh
    Walsh, Thomas J.
    Jensen, Gerard M.
    Hope, William W.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (08) : 3432 - 3441
  • [9] Comparative Pharmacodynamics of Amphotericin B Lipid Complex and Liposomal Amphotericin B in a Murine Model of Pulmonary Mucormycosis
    Lewis, Russell E.
    Albert, Nathan D.
    Liao, Guangling
    Hou, Jingguo
    Prince, Randall A.
    Kontoyiannis, Dimitrios P.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (03) : 1298 - 1304
  • [10] EFFECT OF ANTIBODIES ON ELECTROCONDUCTIVITY OF ION CHANNELS FORMED BY AMPHOTERICIN-B IN BIMOLECULAR LIPID-MEMBRANE
    KOLOMYTKIN, OV
    BIOLOGICHESKIE MEMBRANY, 1986, 3 (06): : 609 - 620