Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

被引：36

作者：

Wang, Jianchang ^{[1
]}

Liu, Libing ^{[1
]}

Li, Ruiwen ^{[2
]}

Wang, Jinfeng ^{[1
]}

Fu, Qi ^{[1
]}

Yuan, Wanzhe ^{[2
]}

机构：

[1] Hebei Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Tech Ctr, 318 Heping West Rd, Shijiazhuang 050051, Hebei, Peoples R China

[2] Agr Univ Hebei, Coll Vet Med, 38 Lingyusi St, Baoding 071001, Hebei, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2016年 / 161卷 / 04期

关键词：

Canine parvovirus type 2; Molecular diagnosis; Recombinase polymerase amplification; Polymerase chain reaction; CHAIN-REACTION; INFECTION; ENTERITIS; VIRUS; ASSAY; DOGS; DNA;

D O I：

10.1007/s00705-015-2738-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.

引用

页码：1015 / 1018

页数：4

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