Organochloride pesticides modulated gut microbiota and influenced bile acid metabolism in mice

被引:70
|
作者
Liu, Qian [1 ,2 ,3 ]
Shao, Wentao [2 ,3 ]
Zhang, Chunlan [2 ,3 ]
Xu, Cheng [2 ,3 ]
Wang, Qihan [4 ]
Liu, Hui [2 ,3 ]
Sun, Haidong [4 ]
Jiang, Zhaoyan [1 ]
Gu, Aihua [2 ,3 ]
机构
[1] Tongji Univ, Sch Med, Inst Gallstone Dis, Ctr Gallbladder Dis,Shanghai East Hosp, Shanghai, Peoples R China
[2] Nanjing Med Univ, Inst Toxicol, State Key Lab Reprod Med, Nanjing, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Sch Publ Hlth, Minist Educ, Key Lab Modern Toxicol, Nanjing, Jiangsu, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Surg,Shanghai Inst Digest Surg, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
Organochloride pesticides; Bile acids metabolism; Intestinal microbiota; BETA-MURICHOLIC ACID; INTESTINAL MICROBIOTA; CHOLESTEROL ABSORPTION; EXPOSURE; MECHANISMS; DISEASE;
D O I
10.1016/j.envpol.2017.03.068
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Organochlorine pesticides (OCPs) can persistently accumulate in body and threaten human health. Bile acids and intestinal microbial metabolism have emerged as important signaling molecules in the host. However, knowledge on which intestinal microbiota and bile acids are modified by OCPs remains unclear. In this study, adult male C57BL/6 mice were exposed to p, p'-dichlorodiphenyldichloroethylene (p, p'-DDE) and 8-hexachlorocyclohexane (beta-HCH) for 8 weeks. The relative abundance and composition of various bacterial species were analyzed by 16S rRNA gene sequencing. Bile acid composition was analyzed by metabolomic analysis using UPLC-MS. The expression of genes involved in hepatic and enteric bile acids metabolism was measured by real-time PCR. Expression of genes in bile acids synthesis and transportation were measured in HepG2 cells incubated with p, p'-DDE and beta-HCH. Our findings showed OCPs changed relative abundance and composition of intestinal, microbiota, especially in enhanced Lactobacillus with bile salt hydrolase (BSH) activity. OCPs affected bile acid composition, enhanced hydrophobicity, decreased expression of genes on bile acid reabsorption in the terminal ileum and compensatory increased expression of genes on synthesis of bile acids in the liver. We demonstrated that chronic exposure of OCPs could impair intestinal microbiota; as a result, hepatic and enteric bile acid profiles and metabolism were influenced. The findings in this study draw our attention to the hazards of chronic OCPs exposure in modulating bile acid metabolism that might cause metabolic disorders and their potential to cause related diseases in human. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:268 / 276
页数:9
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