Serum Ceruloplasmin Oxidase Activity and Malondialdehyde Level in Experimental Abdominal Compartment Syndrome-Induced Rats

Objective: The aim of this study was to investigate serum ceruloplasmin (Cp) oxidase activity and malondialdehyde (MDA) levels in a rat model of abdominal compartment syndrome (ACS). Material and Methods: There were four groups consisting of eight rats per group. Group 1 (Control group): After anesthesia, 3 ml of blood were taken from the heart of these rats. Group 2 (Sham group): Under anesthesia, an injector needle was inserted intraperitoneally and left there. After 3 h, 3 ml of blood was taken from the heart of these rats. Group 3 (First study group): Under anesthesia, an injector needle (number 16) was inserted intraperitoneally and a standard insufflator (Karl Storz, Germany) was connected. At the end of this procedure, the abdomen was decompressed and 3 ml of blood was collected as descried above. Group 4 (Second study group): The abdomen was compressed as Group 3. However, the intraabdominal hypertension was established by keeping the pressure constant at 25 mmHg for 3 h in this group. At the end of this procedure, the abdomen was decompressed and 3 ml of blood was collected as descried above. Results: Cp oxidase activities and MDA levels significantly increased in first and second study groups when compared to the control group. Cp oxidase activities and MDA levels were significantly higher in second study group than those of the first study group. MDA levels were significantly higher in first study group than those of the sham group. Conclusion: We found increased serum Cp oxidase activity and MDA level in experimental abdominal compartment syndrome-induced rats. These findings show that oxidative stress increase ACS in rats.