Novel Method to Detect a Construct-Specific Sequence of the Acetolactate Synthase Gene in Genetically-Modified Flax CDC Triffid (FP967)

被引：12

作者：

Nakamura, Kosuke ^{[1
]}

Akiyama, Hiroshi ^{[1
]}

Yamada, Chihiro ^{[2
]}

Satoh, Rie ^{[1
]}

Makiyama, Daiki ^{[1
]}

Sakata, Kozue ^{[1
]}

Kawakami, Hiroshi ^{[2
]}

Mano, Junichi ^{[3
]}

Kitta, Kazumi ^{[3
]}

Teshima, Reiko ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Setagaya Ku, Tokyo 1588501, Japan

[2] Kyoritsu Womens Univ, Dept Food Sci & Nutr, Chiyoda Ku, Tokyo 1018437, Japan

[3] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2010年 / 33卷 / 03期

关键词：

genetically modified organism; flax; polymerase chain reaction; genomic DNA; LINUM-USITATISSIMUM; RESISTANT;

D O I：

10.1248/bpb.33.532

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was defected in many countries including Japan. A method to reliably identify, the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.

引用

页码：532 / 534

页数：3

共 3 条

[1] Collaborative trial validation of a construct-specific real-time PCR method for detection of genetically modified linseed event 'CDC Triffid' FP967
Grohmann, Lutz
Busch, Ulrich
Pecoraro, Sven
Hess, Norbert
Pietsch, Klaus
Mankertz, Joachim
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2011, 232 (03) : 557 - 561
[2] Collaborative trial validation of a construct-specific real-time PCR method for detection of genetically modified linseed event ‘CDC Triffid’ FP967
Lutz Grohmann
Ulrich Busch
Sven Pecoraro
Norbert Hess
Klaus Pietsch
Joachim Mankertz
European Food Research and Technology, 2011, 232 : 557 - 561
[3] Development of an event-specific assay for the qualitative and quantitative detection of the genetically modified flax CDC Triffid (FP967)
Vanella, Rosario
Weston, Anna
Brodmann, Peter
Kuebler, Eric
FOOD CONTROL, 2014, 41 : 128 - 133

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