In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope

被引:4
|
作者
Qin, Xiaofei [1 ]
Chen, Chong [2 ]
Wang, Linbo [2 ]
Chen, Xiaohu [2 ]
Liang, Yong [2 ]
Jin, Xin [2 ]
Pan, Weijun [3 ]
Liu, Zhiying [1 ]
Li, Hui [1 ,2 ]
Yang, Guang [2 ]
机构
[1] Changchun Univ Sci & Technol, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Jiangsu, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai 200031, Peoples R China
基金
中国国家自然科学基金;
关键词
Zebrafish intersegmental vessel; Light-sheet illumination microscope; Maximum intensity projection; Virtual reality;
D O I
10.1016/j.bbrc.2021.03.160
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed.(c) 2021 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:8 / 13
页数:6
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