Pontibaca salina sp. nov., isolated from marine sediment

A Gram-stain-negative, oval or short rod-shaped, non-motile, aerobic bacterium, designated strain S1109L(T), was isolated from a marine sediment in Weihai, PR China. Cells were oxidase positive and catalase positive. Growth of strain S1109L(T) occurred at 10-40 degrees C (optimum, 30-33 degrees C), pH 6.5-10.0 (optimum, 7.0-8.0) and in the presence of 1-21% (optimum, 4-6%) (w/v) NaCl. 16S rRNA gene sequence phylogeny indicated that strain S1109L(T) was associated with the genus Pontibaca of the family Rhodobacteraceae because it showed the highest sequence similarity to Pontibaca methylaminivorans KCTC 22497(T) (97.5%). The average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) scores between strain S1109L(T) and Pontibaca methylaminivorans KCTC 22497(T) were 74.6% and 18.7%. The major cellular fatty acids of strain S1109L(T) were C-19:0 cyclo omega 8c and C-18:1 omega 7c. The polar lipids profiles of strain S1109L(T) were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. Strain S1109L(T) contained ubiquinone-10 as the major respiratory quinone. The genomic DNA G + C content was 55.9 mol%. On the basis of the evidence presented in this study, strain S1109L(T) is considered to represent a novel species of the genus Pontibaca, for which the name Pontibaca salina sp. nov. is proposed. The type strain of is S1109L(T) (= KCTC 82411(T) = MCCC 1H00441(T)).