Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

被引：12

作者：

Kinahan, David J. ^{[1
]}

Mangwanya, Faith ^{[1
]}

Garvey, Robert ^{[1
]}

Chung, Danielle W. Y. ^{[1
]}

Lipinski, Artur ^{[1
]}

Julius, Lourdes A. N. ^{[1
]}

King, Damien ^{[1
]}

Mohammadi, Mehdi ^{[1
]}

Mishra, Rohit ^{[1
]}

Al-Ofi, May ^{[1
]}

Miyazaki, Celina ^{[1
]}

Ducree, Jens ^{[1
]}

机构：

[1] Dublin City Univ, Natl Ctr Sensor Res, Sch Phys Sci, Dublin, Ireland

来源：

27TH MICROMECHANICS AND MICROSYSTEMS EUROPE WORKSHOP (MME 2016) | 2016年 / 757卷

基金：

爱尔兰科学基金会;

关键词：

WHOLE-BLOOD; MICROFLUIDICS; PURIFICATION; INTEGRATION;

D O I：

10.1088/1742-6596/757/1/012013

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

引用

页数：6

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