Opposite actions of urotensin II and relaxin-2 on cellular expression of fibronectin in renal fibrosis: A preliminary experimental study

被引：3

作者：

Cernaro, Valeria ^{[1
]}

Medici, Maria A. ^{[2
]}

Bianco, Federica ^{[2
]}

Santoro, Domenico ^{[1
]}

Lacquaniti, Antonio ^{[1
]}

Romeo, Adolfo ^{[1
]}

Lucisano, Silvia ^{[1
]}

Buemi, Antoine ^{[1
]}

Buemi, Michele ^{[1
]}

机构：

[1] Univ Messina, Chair Nephrol, Dept Clin & Expt Med Univ, Via Consolare Valeria 1, I-98124 Messina, Italy

[2] Univ Messina, Dept Biol & Environm Sci, Messina, Italy

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 2017年 / 44卷 / 10期

关键词：

fibronectin; fibrosis; relaxin-2; urantide; urotensin II; NEONATAL CARDIAC FIBROBLASTS; INDUCED COLLAGEN-SYNTHESIS; TUBULOINTERSTITIAL FIBROSIS; IN-VIVO; PROGRESSION; FAILURE; HYPERTENSION; INVOLVEMENT; DYSFUNCTION; DISEASE;

D O I：

10.1111/1440-1681.12798

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Our aim was to evaluate the role of urotensin II, urantide (urotensin II receptor antagonist) and relaxin-2 on the cellular expression of fibronectin as a surrogate marker for renal fibrosis. We employed LLC-PK1 renal tubular epithelial cells and assessed the influence on the fibrotic process of the above-mentioned substances by using anti-fibronectin antibodies in western blot analysis. The addition of urotensin II increased fibronectin expression. Urantide reduced the positivity for fibronectin caused by urotensin II (P<.05). The anti-fibrotic action was more evident for relaxin-2 (P<.01). Also in the model of TGF-1-induced fibrosis, urantide and, to a greater extent, relaxin-2 were able to significantly lessen fibronectin expression (respectively, P<.05 and P<.01). In conclusion, relaxin-2 may reduce urotensin II-induced renal fibrosis.

引用

页码：1069 / 1071

页数：3