Development of molecular markers for high oleic acid content in sunflower (Helianthus annuus L.)

被引：12
|
作者：
Dehmer, KJ [1 ]
Friedt, W [1 ]
机构：
[1] Univ Giessen, Inst Crop Sci & Plant Breeding, D-35390 Giessen, Germany
来源：
INDUSTRIAL CROPS AND PRODUCTS | 1998年 / 7卷 / 2-3期
关键词：
Helianthus annuus; high oleic; OL1; bulked segregant analysis; AP-PCR; RAPDs;
D O I：
10.1016/S0926-6690(97)00063-0
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In order to achieve the development of molecular markers for the 'high oleic' trait in sunflower (Helianthus annuus L.), F-2 populations segregating for this trait were examined. One of the respective populations exhibited a 3:1 segregation ratio for high oleic acid content, being the result of only one gene segregating and thus well suited for molecular analyses. Employing the 'bulked segregant analysis' in combination with PCR experiments, two primers (OP-AC10, OP-F15) generated differentiating bands (AC10-765, F15-690) between the low oleic and high oleic bulks, as well as between the respective F, individuals. Linkage analysis using: the 'Mapmaker' software resulted in genetic distances of 7.2 cM and 7.0 cM to the OL1 locus, respectively. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：311 / 315
页数：5
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