An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces

被引：44
|
作者：
Rodák, L [1 ]
Valícek, L [1 ]
Smíd, B [1 ]
Nevoránková, Z [1 ]
机构：
[1] Vet Res Inst, Dept Virol, CS-62132 Brno, Czech Republic
来源：
VETERINARY MICROBIOLOGY | 2005年 / 105卷 / 01期
关键词：
porcine epidemic diarrhoea virus (PEDV); blocking ELISA; viral gastroenteritis of swine; monoclonal antibodies;
D O I：
10.1016/j.vetmic.2004.09.020
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined. and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：9 / 17
页数：9
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