Description of Polaribacter aestuariivivens sp. nov., isolated from a tidal flat

A bacterial strain, DBTF-3(T), was isolated from a tidal flat of Republic of Korea. Phylogenetic trees of 16S rRNA gene sequences showed that strain DBTF-3(T) belonged to the genus Polaribacter. Strain DBTF-3(T) exhibited 16S rRNA gene sequence similarities of 97.1-98.1% to type strains of P. dokdonensis, P. haliotis, P. marinaquae, P. insulae, P. vadi, P. glomeratus, P. irgensii and P. reichenbachii, and 94.0-96.9% to those of the other Polaribacter species. DNA-DNA relatedness values of strain DBTF-3(T) with type strains of P. marinaquae and P. insulae were 14-19%. Average nucleotide identity and digital DNA-DNA hybridization values between strain DBTF-3(T) and type strains of six other Polaribacter species were 76.5-83.5% and 20.9-27.1%, respectively. Strain DBTF-3(T) contained MK-6 as the predominant menaquinone, and iso-C-15:0, summed feature 3, iso-C-15:1 G and iso-C-15:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain DBTF-3(T) is separated from Polaribacter species. On the basis of the data presented, strain DBTF-3(T) (= KACC 19612(T) = NBRC 113191(T)) represents a novel species of the genus Polaribacter, for which the name Polaribacter aestuariivivens sp. nov. is proposed.