albumin;
protein adsorption;
protein conformation;
joint replacement;
arthroplasty;
friction;
interface;
polyethylene;
hydrophilicity;
D O I:
10.1016/j.biomaterials.2004.05.020
中图分类号:
R318 [生物医学工程];
学科分类号:
0831 ;
摘要:
Wear of articulated surfaces can be a major lifetime-limiting factor in arthroplasty. In the natural joint, lubrication is effected by the body's natural synovial fluid. Following arthroplasty, and the subsequent reformation of the synovial membrane, a fluid of similar composition surrounds the artificial joint. Synovial fluid contains, among many other constituents, a substantial concentration of the readily adsorbing protein albumin. The ability of human serum albumin to act as a boundary lubricant in joint prostheses has been investigated using a pin-on-disc tribometer. Circular dichroism spectroscopy was employed to follow the temperature- and time-dependent conformational changes of human serum albumin in the model lubricant solution. Effects of protein conformation and polymer surface hydrophilicity on protein adsorption and the resulting friction in the boundary lubrication regime have been investigated. Unfolded proteins preferentially adsorb onto hydrophobic polymer surfaces, where they form a compact, passivating layer and increase sliding friction-an effect that can be largely suppressed by rendering the substrate more hydrophilic. A molecular model for protein-mediated boundary friction is proposed to consolidate the observations. The relevance of the results for in vivo performance and ex vivo hip-joint testing are discussed. (C) 2004 Elsevier Ltd. All rights reserved.
机构:
Tel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
Lagziel-Simis, Shira
Cohen-Hadar, Noa
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机构:
Tel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
Cohen-Hadar, Noa
Moscovich-Dagan, Hila
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机构:
Tel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
Moscovich-Dagan, Hila
Wine, Yariv
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h-index: 0
机构:
Tel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
Wine, Yariv
Freeman, Amihay
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h-index: 0
机构:
Tel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
机构:
Univ So Calif, Ctr Craniofacial Mol Biol, Ostrow Sch Dent, Los Angeles, CA 90033 USAUniv So Calif, Ctr Craniofacial Mol Biol, Ostrow Sch Dent, Los Angeles, CA 90033 USA
Moradian-Oldak, Janet
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