Alginate gel cell microspheres combined with biphasic calcium phosphate ceramics to form a dual 3D programmed culture system for bone regeneration

Low cell viability, cell survival rate and unsatisfactory vascularization ability have always been a great challenge for the application of tissue engineered bone. Building a 3D culture system to simulate the micro-environment of seed cells may be a potential method to improve the cell viability, cell survival rate and vascularization ability of tiusse engineered bone. Thus, based on our previous work, we aimed to build a dual 3D programmed culture system for bone regeneration. Stem cell alginate beads were seeded into biphasic calcium phosphate ceramics to form a dual 3D programmed culture system. The content of the culture medium and the growth factors were adjusted based on the expression levels of osteogenesis and angiogenesis related growth factors. This dual 3D culture system was estimated by in vitro and in vivo experiment. The results indicated that alginate gel cell microspheres combined with BCPCs stimulated the regeneration of bone and vessels more effectively than other groups. Our research provides new experimental evidence and a theoretical basis for clinical application of this dual 3D culture system.