Artifact-free high-density localization microscopy analysis

被引：71
|
作者：
Marsh, Richard J. [1 ]
Pfisterer, Karin [1 ]
Bennett, Pauline [1 ]
Hirvonen, Liisa M. [1 ]
Gautel, Mathias [1 ]
Jones, Gareth E. [1 ]
Cox, Susan [1 ]
机构：
[1] Kings Coll London, Randall Ctr Cell & Mol Biophys, London, England
来源：
NATURE METHODS | 2018年 / 15卷 / 09期
基金：
英国生物技术与生命科学研究理事会;
关键词：
IMMUNOELECTRON MICROSCOPY; TITIN; STORM; NANOSCOPY; MUSCLE; IMAGEJ;
D O I：
10.1038/s41592-018-0072-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling highspeed, artifact-free super-resolution imaging of live cells.
引用
收藏
页码：689 / +
页数：7
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