Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis

被引:71
|
作者
Park, Kyu-Ho
Berrier, Catherine
Lebaupain, Florence
Pucci, Bernard
Popot, Jean-Luc
Ghazi, Alexandre
Zito, Francesca [1 ]
机构
[1] CNRS, UMR 7099, Lab Physicochim Mol Membranes Biol, F-75005 Paris, France
[2] Univ Paris 07, CNRS, Inst Biol Physicochim, FRC 550, F-75005 Paris, France
[3] CNRS, UMR 8619, Inst Biochim & Biophys Mol & Cellulaire, F-91405 Orsay, France
[4] Univ Paris 11, F-91405 Orsay, France
[5] Univ Avignon & Pays Vaucluse, Lab Chim Bioorgan & Syst Mol Vectoriels, F-84000 Avignon, France
关键词
detergent; fluorinated surfactant; hemifluorinated surfactant; membrane delivery; membrane protein; MscL;
D O I
10.1042/BJ20061473
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hemifluorinated and fluorinated surfactants are lipophobic and, as such, non-detergent. Although they do not solubilize biological membranes, they can, after conventional solubilization, substitute for detergents to keep membrane proteins soluble, which generally improves their stability [Breyton, Chabaud, Chaudier, Pucci and Popot (2004) FEBS Lett. 564, 312-318]. In the present study, we show that (hemi)fluorinated surfactants can be used for in vitro synthesis of membrane proteins: they do not interfere with protein synthesis, and they provide a suitable environment for MscL, a pentameric mechanosensitive channel, to fold and oligomerize to its native functional state. Following synthesis, both types of surfactants can be used to deliver MscL directly to pre-formed lipid vesicles. The electrophysiological activity of MscL synthesized in vitro in the presence of either hemi- or per-fluorinated surfactant is similar to that of the protein expressed in vivo.
引用
收藏
页码:183 / 187
页数:5
相关论文
共 50 条
  • [22] A microfabricated reactor for cell-free protein synthesis
    Fujii, T
    Hosokawa, K
    Nojima, T
    Shoji, S
    Yotsumoto, A
    Endo, I
    PROCEEDINGS OF THE 19TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOL 19, PTS 1-6: MAGNIFICENT MILESTONES AND EMERGING OPPORTUNITIES IN MEDICAL ENGINEERING, 1997, 19 : 2572 - 2574
  • [23] Cell-free protein synthesis in hydrogel materials
    Whitfield, Colette J.
    Banks, Alice M.
    Dura, Gema
    Love, John
    Fieldsend, Jonathan E.
    Goodchild, Sarah A.
    Fulton, David A.
    Howard, Thomas P.
    CHEMICAL COMMUNICATIONS, 2020, 56 (52) : 7108 - 7111
  • [24] ALVEOLAR CELLS - CELL-FREE PROTEIN SYNTHESIS
    MASSARO, D
    AMERICAN REVIEW OF RESPIRATORY DISEASE, 1967, 96 (01): : 158 - &
  • [25] Methods for energizing cell-free protein synthesis
    Kim, Ho-Cheol
    Kim, Dong-Myung
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 108 (01) : 1 - 4
  • [26] Cell-free soluble expression of the membrane protein PsbS
    Krishnan, M.
    de Leeuw, T. J. J. F.
    Pandit, A.
    PROTEIN EXPRESSION AND PURIFICATION, 2019, 159 : 17 - 20
  • [27] Cell-free protein synthesis: the state of the art
    Whittaker, James W.
    BIOTECHNOLOGY LETTERS, 2013, 35 (02) : 143 - 152
  • [28] Cell-free protein synthesis in a microfabricated reactor
    Nojima, T
    Fujii, T
    Hosokawa, K
    Yotsumoto, A
    Shoji, S
    Endo, I
    BIOPROCESS ENGINEERING, 2000, 22 (01) : 13 - 17
  • [29] CELL-FREE PROTEIN SYNTHESIS IN MAMMALIAN SKIN
    FREEDBERG, IM
    FINE, IH
    CORDELLE, FH
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1967, 48 (01) : 55 - +
  • [30] ALVEOLAR MACROPHAGES - CELL-FREE PROTEIN SYNTHESIS
    MASSARO, D
    AMERICAN REVIEW OF RESPIRATORY DISEASE, 1969, 100 (02): : 249 - &