Aberrant expression of a five-microRNA signature in breast carcinoma as a promising biomarker for diagnosis

被引:29
|
作者
Bitaraf, Amirreza [1 ]
Babashah, Sadegh [1 ]
Garshasbi, Masoud [2 ]
机构
[1] Tarbiat Modares Univ, Fac Biol Sci, Dept Mol Genet, POB 14115-154, Tehran, Iran
[2] Tarbiat Modares Univ, Fac Med Sci, Dept Med Genet, Tehran, Iran
关键词
biomarker; breast cancer; diagnosis; HER-2; microRNA; ONCOGENIC ROLE; CANCER; MICRORNA; PROLIFERATION; MIR-199B-5P; PREDICTION; PROGNOSIS; MIR-133A; MIR-21; ROLES;
D O I
10.1002/jcla.23063
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background Breast cancer (BC) is the most common malignancy among females with dismal quality of life in patients. It has been proven that epigenetic factors, especially microRNAs, are involved in breast carcinogenesis and progression. This study aimed to assess the expression and clinical performances of a five-microRNA signature (miR-127-3p, miR-133a-3p, miR-155-5p, miR-199b-5p, and miR-342-5p) in breast cancer and adjacent normal tissues to identify a potential biomarker for BC and investigate the relationship between their expression and clinicopathological features of BC patients as well. Methods In this case-control investigation, we recruited 50 pairs of tumor and matched non-tumor surgical specimens from patients diagnosed with BC. Expression levels of miR-127-3p, miR-133a-3p, miR-155-5p, miR-199b-5p, and miR-342-5p were measured in BC and adjacent normal tissues by RT-qPCR. Results We found that miR-127-3p, miR-133a-3p, miR-199b-5p, and miR-342-5p were significantly down-regulated, while miR-155-5p was significantly up-regulated in BC tumor tissues compared with the corresponding adjacent normal tissues. The decreased expression of miR-127-3p, miR-133a-3p, miR-342-5p, and up-regulation of miR-155-5p showed a significant correlation with disease stage. We also found a significant down-regulation of miR-127-3p, miR-199b-5p, and miR-342-5p compared in HER-2-negative patients. Our results indicated that miR-155-5p had a higher expression level in HER-2-positive patients. Receiver operating characteristic (ROC) curve analysis demonstrated that all these five microRNAs can serve as potential biomarkers to distinguish between tumor and non-tumor breast tissue samples. Conclusions The present findings suggested that dysregulation of this five-miRNA signature might be considered as a promising and functional biomarker for BC diagnosis.
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页数:8
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